Ferré N, Camps J, Paul A, Cabré M, Calleja L, Osada J, Joven J
Centre de Recerca Biomèdica, Hospital Universitari de Sant Joan, Cantalunya, Spain.
Mol Cell Biochem. 2001 Feb;218(1-2):165-9. doi: 10.1023/a:1007296919243.
The present study describes the effects of several high-fat low-cholesterol antiatherogenic diets on the hepatic lipid peroxidation and hepatic antioxidant systems in apolipoprotein E-deficient mice. Eighty mice were distributed into five groups and fed with regular mouse chow or chow supplemented with coconut, palm, olive and sunflower seed oils. After ten weeks, they were sacrificed and the livers were removed so that lipid peroxidation and alpha-tocopherol concentrations, and superoxide dismutase, glutathione peroxidase and glutathione reductase activities could be measured. The size of the atherosclerotic lesions in the aortas was also measured. Results showed that the diets supplemented with olive oil, palm oil or sunflower seed oil significantly decreased the size of the lesion. However, there was an association between those mice that were on diets supplemented with palm or coconut oils and a significant increase in hepatic lipid peroxidation. This association was not found in animals fed with olive or sunflower seed oils, the diets with the highest content of vitamin E. The dietary content of vitamin E was significantly correlated (r = 0.98; p < 0.05) with the hepatic concentration of this compound. Our study suggests that the high content of vitamin E in olive oil or sunflower seed oil may protect from the undesirable hepatotoxic effects of high-fat diets in apo E-deficient mice and that this should be taken into account when these diets are used to prevent atherosclerosis.
本研究描述了几种高脂肪低胆固醇抗动脉粥样硬化饮食对载脂蛋白E缺乏小鼠肝脏脂质过氧化和肝脏抗氧化系统的影响。将80只小鼠分为五组，分别喂食常规小鼠饲料或添加椰子油、棕榈油、橄榄油和葵花籽油的饲料。十周后，将它们处死并取出肝脏，以便测量脂质过氧化和α-生育酚浓度，以及超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活性。还测量了主动脉中动脉粥样硬化病变的大小。结果表明，添加橄榄油、棕榈油或葵花籽油的饮食显著减小了病变的大小。然而，食用添加棕榈油或椰子油饲料的小鼠与肝脏脂质过氧化显著增加之间存在关联。在喂食橄榄油或葵花籽油（维生素E含量最高的饮食）的动物中未发现这种关联。饮食中维生素E的含量与该化合物在肝脏中的浓度显著相关（r = 0.98；p < 0.05）。我们的研究表明，橄榄油或葵花籽油中高含量的维生素E可能保护载脂蛋白E缺乏小鼠免受高脂肪饮食不良的肝毒性影响，并且在使用这些饮食预防动脉粥样硬化时应考虑到这一点。
