Salam Karim Y, Hachim S K, Abdul Ali A, Ameen Baqer A, Ali Yaseen M, Lafta H A, Hussein Adhab Z, Ayad Kareem H, Shaker Hamza I, Hamad D A
Department of Pharmacy, Al-Manara College for Medical Sciences, Maysan, Iraq.
College of Technical Engineering, The Islamic University, Najaf, Iraq.
Arch Razi Inst. 2022 Jun 30;77(3):1165-1171. doi: 10.22092/ARI.2022.358740.2298. eCollection 2022 Jun.
It is well documented that choline is known as one of the essential ingredients of phospholipids. Choline acts as a determinative element for appropriate cell membrane functions. On the other hand α-tocopherol (Vit E) is a fat-soluble vitamin. This vitamin acts as a strong antioxidant in the living body's defense system against oxidative stress. Lipid peroxidation in peripartum and early lactating cows is significantly increased while the level of serum Vit E is decreases dramatically. These concomitant physiological changes demonstrate a higher level of oxidative stress subsequently leads to serious health issues in dairy cows. Therefore, the present research was designed to investigate the following items in dairy cattle: 1) evaluation of the possible changes in serum protein fractions, and 2) comparing the oxidative status of orally RPC and vitamin E supplementation in dairy cows in early lactation period. In the current study 30 early lactating primiparous and multiparous Holstein cows (body condition score (BCS)=2.51 ± 0.10) were used beginning five weeks postpartum. All the animals were randomly divided in to three groups (n=10) (number of lactation=2.61). The animals were randomly assigned to receive one of the following treatments. Group 1 served as control group were not received any supplement. The second group was supplemented with 90 g/d of RPC (Reashre Choline, Balchem, USA). The third group was administrated 4400 IU/d vitamin E (Roche, Vitamins Ltd; Switzerland). In the current study, serum protein electrophoresis showed four main fractions as follows: albumin, α-globulin, β-globulin, and γ-globulin. The recorded data showed that the percentages of albumin and γ-globulin fractions were higher in treated groups compared to the control group. In the animals supplementing with RPC and vitamin E the percentages of serum albumin increased to the value of 37. 70±1.63 and 38.21±1.28 respectively compare to the control group (34.69±1.21), which were significant (<0.05).
有充分文献记载，胆碱是磷脂的必需成分之一。胆碱是细胞膜正常功能的决定性元素。另一方面，α-生育酚（维生素E）是一种脂溶性维生素。这种维生素在生物体抵御氧化应激的防御系统中作为一种强大的抗氧化剂发挥作用。围产期和早期泌乳奶牛的脂质过氧化显著增加，而血清维生素E水平则急剧下降。这些伴随的生理变化表明，更高水平的氧化应激随后会导致奶牛出现严重的健康问题。因此，本研究旨在调查奶牛的以下项目：1）评估血清蛋白组分可能的变化，以及2）比较早期泌乳期奶牛口服RPC和维生素E补充剂后的氧化状态。在本研究中，从产后五周开始使用30头早期泌乳的初产和经产荷斯坦奶牛（体况评分（BCS）=2.51±0.10）。所有动物随机分为三组（n=10）（泌乳次数=2.61）。动物被随机分配接受以下治疗之一。第1组作为对照组，不接受任何补充剂。第二组补充90克/天的RPC（Reashre胆碱公司，美国巴尔克姆）。第三组给予4400国际单位/天的维生素E（罗氏维生素有限公司，瑞士）。在本研究中，血清蛋白电泳显示出以下四个主要组分：白蛋白、α-球蛋白、β-球蛋白和γ-球蛋白。记录的数据表明，与对照组相比，治疗组中白蛋白和γ-球蛋白组分的百分比更高。在补充RPC和维生素E的动物中，血清白蛋白的百分比分别增加到37.70±1.63和38.21±1.28，与对照组（34.69±1.21）相比有显著差异（<0.05）。
