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小鼠下丘脑第三脑室室管膜上结构及室管膜的表面精细结构

Surface fine structure of supraependymal elements and ependyma of hypothalamic third ventricle of mouse.

作者信息

Bleier R

出版信息

J Comp Neurol. 1975 Jun 15;161(4):555-67. doi: 10.1002/cne.901610406.

DOI:10.1002/cne.901610406
PMID:1133231
Abstract

The ependymal surface of the hypothalamic third ventricle of the adult female and male mouse was examined with the scanning electron microscope. The material was dried with the critical point method. Supraependymal cells, exhibiting a variety of forms, lie among the cilia of the rostrodorsal two-thirds of the ventricle. The cell bodies are usually between 8 and 10 mu in diameter and have dividing pseudopodial processes which may be broad or narrow, flat or stout, smooth or varicosed. The textured surfaces of the cells seen in this material appear to have no cilia, microvilli or membraneous folds though they may have small bleb-like protrusions. Some cells and adjacent cilia bear clusters of friable-looking material that may be secretory. In several female mice, mounds of debris, that could be degenerating cells or secretory material, were seen in intimate association with clusters of cells. The cells' processes extend over and through the surface of the mound. While some supraependymal cells may be neurons or glia, it is suggested that many of the cells seen may be morphological variants of one cell line, mononuclear phagocytes. Sausage-shaped structures at the end of long cilia-like stalks are described and their possible identity discussed.

摘要

用扫描电子显微镜对成年雌性和雄性小鼠下丘脑第三脑室的室管膜表面进行了检查。材料采用临界点干燥法进行干燥处理。室管膜上细胞形态多样,位于脑室前三分之二背侧的纤毛之间。细胞体直径通常在8至10微米之间,具有可分裂的伪足突起,这些突起可能宽或窄、扁平或粗壮、光滑或曲张。在这种材料中观察到的细胞表面纹理似乎没有纤毛、微绒毛或膜状褶皱,不过它们可能有小泡状突起。一些细胞和相邻的纤毛带有看起来易碎的物质簇,可能具有分泌功能。在几只雌性小鼠中,发现与细胞簇紧密相连的碎片堆,这些碎片可能是正在退化的细胞或分泌物质。细胞的突起延伸到碎片堆表面并贯穿其中。虽然一些室管膜上细胞可能是神经元或神经胶质细胞,但有人认为所观察到的许多细胞可能是单核吞噬细胞这一细胞系的形态变体。文中描述了长纤毛状柄末端的香肠形结构,并对其可能的身份进行了讨论。

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