维生素B12b和C联合使用会导致人喉表皮样癌细胞HEp-2的谷胱甘肽耗竭和细胞死亡。

Combined vitamins Bl2b and C induce the glutathione depletion and the death of epidermoid human larynx carcinoma cells HEp-2.

作者信息

Akatov V S, Evtodienko Y V, Leshchenko V V, Teplova V V, Potselueva M M, Kruglov A G, Lezhnev E I, Yakubovskaya R I

机构信息

Laboratory of Cytotechnology, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region.

出版信息

Biosci Rep. 2000 Oct;20(5):411-7. doi: 10.1023/a:1010386102562.

DOI:10.1023/a:1010386102562

PMID:11332602

Abstract

The combination of hydroxocobalamin (vitamin B12b) and ascorbic acid (vitamin C) can cause the death of tumor cells at the concentrations of the components at which they are nontoxic when administered separately. This cytotoxic action on epidermoid human larynx carcinoma cells HEp-2 in vitro is shown to be due to the hydrogen peroxide generated by the combination of vitamins B12b and C. The drop in the glutathione level preceding cell death was found to be the result of combined action of the vitamins. It is supposed that the induction of cell death by combined action of vitamins B12b and C is connected to the damage of the cell redox system.

摘要

羟钴胺素（维生素B12b）和抗坏血酸（维生素C）联合使用时，在各成分单独给药无毒的浓度下可导致肿瘤细胞死亡。体外实验表明，这种对人喉表皮样癌细胞HEp-2的细胞毒性作用是由于维生素B12b和C联合产生的过氧化氢所致。细胞死亡前谷胱甘肽水平的下降是维生素联合作用的结果。据推测，维生素B12b和C联合作用诱导细胞死亡与细胞氧化还原系统的损伤有关。