小麦黄斑叶枯病菌ToxB基因的特性分析
Characterization of the ToxB gene from Pyrenophora tritici-repentis.
作者信息
Martinez J P, Ottum S A, Ali S, Franci L J, Ciuffetti L M
机构信息
Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331, USA.
出版信息
Mol Plant Microbe Interact. 2001 May;14(5):675-7. doi: 10.1094/MPMI.2001.14.5.675.
PMID:11332732
Abstract
The ToxB gene was cloned and characterized from a race 5 isolate of Pyrenophora tritici-repentis from North Dakota. ToxB contains a 261-bp open reading frame that encodes a 23 amino acid putative signal peptide and a 64 amino acid host-selective toxin, Ptr ToxB. Analysis of Ptr ToxB from heterologous expression in Pichia pastoris confirms that ToxB encodes a host-selective toxin.
摘要
从北达科他州的小麦根腐病菌5号生理小种分离物中克隆并鉴定了ToxB基因。ToxB包含一个261 bp的开放阅读框，编码一个23个氨基酸的假定信号肽和一个64个氨基酸的寄主选择性毒素Ptr ToxB。对毕赤酵母中异源表达的Ptr ToxB的分析证实，ToxB编码一种寄主选择性毒素。
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