Yang Z H, Rendon A, Flores A, Medina R, Ijaz K, Llaca J, Eisenach K D, Bates J H, Villarreal A, Cave M D
Regional Tuberculosis Genotyping Laboratory, Central Arkansas Veterans Healthcare System, Little Rock 72205, USA.
Int J Tuberc Lung Dis. 2001 Apr;5(4):313-20.
A tuberculosis clinic associated with a university hospital in Monterrey, Mexico, an urban community with high tuberculosis incidence.
To determine the diversity of DNA fingerprint patterns and the extent of drug resistance of Mycobacterium tuberculosis isolates from patients who attended the clinic.
Isolates of M. tuberculosis obtained from 186 patients during the period from 31 January 1996 to 31 March 1998 were tested for susceptibility to isoniazid, rifampicin, ethambutol and streptomycin. Demographic data and the social history of each patient were obtained prospectively by interview. The IS6110 DNA fingerprints were obtained for 166 of the 186 isolates. Secondary typing was carried out on isolates with fewer than six copies of IS6110.
Thirty-two per cent of the tested isolates (60/ 186) were drug-resistant, and 18% (33/186) were multidrug-resistant. Approximately 55% of the resistant isolates (33/60) were attributed to acquired resistance. A total of 106 different IS6110 fingerprint patterns were observed among the 166 fingerprinted isolates. Based on both IS6110 and pTBN12 fingerprinting, 65 (39%) of the 166 isolates were part of 22 DNA fingerprint clusters. Various drug susceptibility patterns were seen in most clusters.
Fingerprint clustering indicates extensive recent transmission of tuberculosis in patients attending the clinic. The prevalence of drug-resistant tuberculosis is high.
墨西哥蒙特雷一所大学医院的结核病诊所，该市社区结核病发病率较高。
确定该诊所患者中结核分枝杆菌分离株的DNA指纹图谱多样性及耐药程度。
对1996年1月31日至1998年3月31日期间从186例患者中获得的结核分枝杆菌分离株进行异烟肼、利福平、乙胺丁醇和链霉素药敏试验。通过访谈前瞻性获取每位患者的人口统计学数据和社会史。对186株分离株中的166株进行IS6110 DNA指纹图谱分析。对IS6110拷贝数少于6个的分离株进行二级分型。
32%（60/186）的受试分离株耐药，18%（33/186）为多重耐药。约55%的耐药分离株（33/60）归因于获得性耐药。在166株有指纹图谱的分离株中，共观察到106种不同的IS6110指纹图谱模式。基于IS6110和pTBN12指纹图谱分析，166株分离株中有65株（39%）属于22个DNA指纹簇。大多数簇中可见多种药敏模式。
指纹聚类表明该诊所就诊患者中近期结核病传播广泛。耐多药结核病患病率较高。
