Cordeiro G M., Casu R, McIntyre C L., Manners J M., Henry R J.
Centre for Plant Conservation Genetics, Southern Cross University, P.O. Box 157, NSW 2480, Lismore, Australia
Plant Sci. 2001 May;160(6):1115-1123. doi: 10.1016/s0168-9452(01)00365-x.
Analysis of a sugarcane (Saccharum spp.) EST (expressed sequence tag) library of 8678 sequences revealed approximately 250 microsatellite or simple sequence repeats (SSRs) sequences. A diversity of dinucleotide and trinucleotide SSR repeat motifs were present although most were of the (CGG)(n) trinucleotide motif. Primer sets were designed for 35 sequences and tested on five sugarcane genotypes. Twenty-one primer pairs produced a PCR product and 17 pairs were polymorphic. Primer pairs that produced polymorphisms were mainly located in the coding sequence with only a single pair located within the 5' untranslated region. No primer pairs producing a polymorphic product were found in the 3' untranslated region. The level of polymorphism (PIC value) in cultivars detected by these SSRs was low in sugarcane (0.23). However, a subset of these markers showed a significantly higher level of polymorphism when applied to progenitor and related genera (Erianthus sp. and Sorghum sp.). By contrast, SSRs isolated from sugarcane genomic libraries amplify more readily, show high levels of polymorphism within sugarcane with a higher PIC value (0.72) but do not transfer to related species or genera well.
对一个包含8678个序列的甘蔗（甘蔗属）EST（表达序列标签）文库进行分析，发现了大约250个微卫星或简单序列重复（SSR）序列。存在多种二核苷酸和三核苷酸SSR重复基序，不过大多数是（CGG）（n）三核苷酸基序。针对35个序列设计了引物组，并在5个甘蔗基因型上进行了测试。21对引物产生了PCR产物，17对具有多态性。产生多态性的引物对主要位于编码序列中，只有一对位于5'非翻译区内。在3'非翻译区未发现产生多态性产物的引物对。这些SSR检测到的甘蔗品种中的多态性水平（PIC值）较低（0.23）。然而，当将这些标记的一个子集应用于祖先种和相关属（斑茅属和高粱属）时，显示出显著更高的多态性水平。相比之下，从甘蔗基因组文库中分离的SSR更容易扩增，在甘蔗中显示出高水平的多态性，PIC值更高（0.72），但向相关物种或属的转移性不佳。
