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转录组特征分析及药用植物分子标记（SNP、SSR 和 InDel）鉴定。

Transcriptome Characterization and Identification of Molecular Markers (SNP, SSR, and Indels) in the Medicinal Plant .

机构信息

College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.

Center of Applied Biotechnology, Wuhan University of Bioengineering, Wuhan 430415, China.

出版信息

Biomed Res Int. 2021 Jul 7;2021:9990910. doi: 10.1155/2021/9990910. eCollection 2021.

DOI:10.1155/2021/9990910
PMID:34307686
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8282378/
Abstract

has significant metabolically active bioingredients of pharmaceutical importance. The deficiency of molecular markers for is a hindrance in molecular breeding for genetic improvement. In this study, 57.756 million pair-end reads were generated by transcriptome sequencing in (Thunb.) Nakai and its subspecies ssp. . A total of 141,954 unigenes with 646.63 bp average length were assembled. A total of 25,620 simple sequence repeats, 726,476 single nucleotide polymorphisms, and 42,939 insertions and deletions were identified, and the associated unigenes and differentially expressed genes were characterized. This work enhanced the molecular marker resources and will facilitate molecular breeding and gene mining in spp.

摘要

具有重要的代谢活性生物成分，具有药物重要性。 缺乏 的分子标记物是遗传改良分子育种的障碍。 在这项研究中，通过转录组测序在 （Thunb.）Nakai 及其亚种 ssp. 中生成了 5775.6 万对末端读长。 总共组装了 141954 个具有 646.63bp 平均长度的基因。 总共鉴定出 25620 个简单序列重复，726476 个单核苷酸多态性和 42939 个插入和缺失，并且对相关的基因和差异表达基因进行了特征描述。 这项工作增强了分子标记资源，将有助于 spp 的分子育种和基因挖掘。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09a3/8282378/d50e5f78b5ff/BMRI2021-9990910.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09a3/8282378/25923a53168f/BMRI2021-9990910.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09a3/8282378/21936bcca852/BMRI2021-9990910.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09a3/8282378/d50e5f78b5ff/BMRI2021-9990910.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09a3/8282378/25923a53168f/BMRI2021-9990910.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09a3/8282378/21936bcca852/BMRI2021-9990910.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09a3/8282378/d50e5f78b5ff/BMRI2021-9990910.003.jpg

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