Dixon W R, Earley J, Postma E
J Pharm Sci. 1975 Jun;64(6):937-9. doi: 10.1002/jps.2600640607.
A simple, rapid, and sensitive radioimmunoassay was developed for the determination of chlordiazepoxide (7-chloro-2-methylamino-5-phenyl-3H-1,4-benzodiazepine 4-oxide) in plasma. Antiserum capable of binding chlordiazepoxide-2-14C was obtained from rabbits following immunization with an antigen prepared by coupling the reactive acyl azide of 7-chloro-5-(4-hydrazinocarbonylmethoxyphenyl)-2-methylamino-3H-1,4-benzodiazepine 4-oxide to bovine serum albumin. The radioimmunoassay allows for the specific determination of chlordiazepoxide directly in plasma without extraction and was compared with a differential spectrofluorometric assay for chlordiazepoxide. Both methods gave satisfactory agreement for the plasma levels of chlordiazepoxide in human subjects resulting from single and chronic oral doses of chlordiazepoxide hydrochloride.
已开发出一种简单、快速且灵敏的放射免疫分析法，用于测定血浆中的氯氮卓（7-氯-2-甲基氨基-5-苯基-3H-1,4-苯并二氮杂卓4-氧化物）。用7-氯-5-(4-肼基羰基甲氧基苯基)-2-甲基氨基-3H-1,4-苯并二氮杂卓4-氧化物的活性酰基叠氮与牛血清白蛋白偶联制备的抗原免疫兔子后，获得了能结合氯氮卓-2-¹⁴C的抗血清。该放射免疫分析法可直接在血浆中特异性测定氯氮卓，无需萃取，并与氯氮卓的差示荧光分光光度法进行了比较。对于单次和长期口服盐酸氯氮卓后人体血浆中氯氮卓的水平，两种方法的测定结果吻合良好。
