Stronjny N, Bratin K, Brooks M A, de Silva J A
J Chromatogr. 1977 Jul 1;143(4):363-74. doi: 10.1016/s0378-4347(00)80982-2.
An analytical procedure was developed for the determination of chlordiazepoxide, diazepam and their major metabolites in blood or plasma. Demoxepam, a metabolite of chlordiazepoxide, is determined by spectrofluorometry after extraction. The remaining compounds are determined by spectrophotodensitometry after thin-layer chromatographic separation. The sensitivity limit of the spectrofluorometric determination of demoxepam is 0.1 to 0.2 microgram while that of the spectrophotodensitometric determination of chlordiazepoxide, diazepam and their N-desmethyl metabolites is 0.05 to 0.2 microgram. The sensitivity and specificity of the assay renders it suitable for monitoring plasma levels of chlordiazepoxide and its major metabolites following single or chronic oral administration of chlordiazepoxide hydrochloride. The sensitivity limit for diazepam and nordiazepam, its major metabolite, renders the assay useful only for the determination of plasma concentrations resulting from high dosage of diazepam. The assay was used to determine chlordiazepoxide and its metabolites following oral administration of Librium. The data showed a significant correlation to those obtained on the same specimens by differential pulse polarography and by radioimmunoassay.
已开发出一种分析方法，用于测定血液或血浆中的氯氮䓬、地西泮及其主要代谢物。去甲地西泮是氯氮䓬的一种代谢物，经萃取后用荧光分光光度法测定。其余化合物经薄层色谱分离后用分光光密度法测定。去甲地西泮荧光分光光度法测定的灵敏度极限为0.1至0.2微克，而氯氮䓬、地西泮及其N-去甲基代谢物分光光密度法测定的灵敏度极限为0.05至0.2微克。该测定方法的灵敏度和特异性使其适用于监测单次或长期口服盐酸氯氮䓬后血浆中氯氮䓬及其主要代谢物的水平。地西泮及其主要代谢物去甲西泮的灵敏度极限使得该测定方法仅适用于测定高剂量地西泮所致的血浆浓度。该测定方法用于测定口服利眠宁后血浆中的氯氮䓬及其代谢物。数据表明，该方法与通过微分脉冲极谱法和放射免疫分析法在相同样本上获得的数据具有显著相关性。
