Robinson-Rechavi M, Marchand O, Escriva H, Bardet P L, Zelus D, Hughes S, Laudet V
Centre National pour la Recherche Scientifique UMR 5665, Laboratoire de Biologie Moléculaire et Cellulaire, Ecole Normale Supérieure de Lyon, 46 Alleé d'Italie 69364 LYON Cedex 07, France.
Genome Res. 2001 May;11(5):781-8. doi: 10.1101/gr.165601.
The presence of additional hox clusters in the zebrafish has led to the hypothesis that there was a whole genome duplication at the origin of modern fish. To investigate the generality of this assumption, we analyzed all available actinopterygian fish gene families, and sequenced nuclear receptors from diverse teleost fish. The origin and timing of duplications was systematically determined by phylogenetic analysis. More genes are indeed found in zebrafish than in mouse. This abundance is shared by all major groups of euteleost fish, but not by eels. Phylogenetic analysis shows that it may result from frequent independent duplications, rather than from an ancestral genome duplication. We predict two zebrafish paralogs for most mouse or human genes, thus expressing a note of caution in functional comparison of fish and mammalian genomes. Redundancy appears to be the rule in fish developmental genetics. Finally, our results imply that the outcome of genome projects cannot be extrapolated easily between fish species.
斑马鱼中额外的hox基因簇的存在引发了这样一种假说，即现代鱼类起源时发生了全基因组复制。为了研究这一假设的普遍性，我们分析了所有可用的辐鳍鱼基因家族，并对多种硬骨鱼的核受体进行了测序。通过系统发育分析系统地确定了复制的起源和时间。确实发现斑马鱼中的基因比小鼠中的更多。所有主要的真骨鱼群都有这种丰富性，但鳗鱼没有。系统发育分析表明，这可能是由于频繁的独立复制，而不是由于祖先基因组复制。我们预测大多数小鼠或人类基因在斑马鱼中有两个旁系同源基因，因此在鱼类和哺乳动物基因组的功能比较中要谨慎。冗余似乎是鱼类发育遗传学中的规律。最后，我们的结果表明，基因组计划的结果不能轻易地在鱼类物种之间外推。
