Hass D, Evans R, Mercenier A, Simon J P, Stalon V
J Bacteriol. 1979 Sep;139(3):713-20. doi: 10.1128/jb.139.3.713-720.1979.
In Pseudomonas aeruginosa arginine can be degraded by the arginine "dihydrolase" system, consisting of arginine deiminase, catabolic ornithine carbamoyltransferase, and carbamate kinase. Mutants of P. aeruginosa strain PAO affected in the structural gene (arcB) of the catabolic ornithine carbamoyltransferase were isolated. Firt, and argF mutation (i.e., a block in the anabolic ornithine carbamoyltransferase) was suppressed specifically by a mutationally altered catabolic ornithine carbamoyltransferase capable of functioning in the anabolic direction. The suppressor locus arcB (Su) was mapped by transduction between hisII and argA. Second, mutants having lost suppressor activity were obtained. The Su- mutations were very closely linked to arcB (Su) and caused strongly reduced ornithine carbamoyltransferase activities in vitro. Under aerobic conditions, a mutant (PA0630) which had less than 1% of the wild-type catabolic ornithine carbamoyltransferase activity grew on arginine as the only carbon and nitrogen source, at the wild-type growth rate. When oxygen was limiting, strain PA0630 grown on arginine excreted citrulline in the stationary growth phase. These observations suggest that during aerobic growth arginine is not degraded exclusively via the dihydrolase pathway.
在铜绿假单胞菌中，精氨酸可被精氨酸“双水解酶”系统降解，该系统由精氨酸脱亚氨酶、分解代谢型鸟氨酸氨甲酰基转移酶和氨基甲酸激酶组成。分离出了在分解代谢型鸟氨酸氨甲酰基转移酶结构基因（arcB）中发生突变的铜绿假单胞菌PAO菌株突变体。首先，一个argF突变（即合成代谢型鸟氨酸氨甲酰基转移酶受阻）被一个能够在合成代谢方向发挥作用的经突变改变的分解代谢型鸟氨酸氨甲酰基转移酶特异性抑制。通过在hisII和argA之间进行转导对抑制基因座arcB（Su）进行了定位。其次，获得了失去抑制活性的突变体。Su-突变与arcB（Su）紧密连锁，并导致体外鸟氨酸氨甲酰基转移酶活性大幅降低。在有氧条件下，一个分解代谢型鸟氨酸氨甲酰基转移酶活性不到野生型1%的突变体（PA0630）以精氨酸作为唯一碳源和氮源，以野生型生长速率生长。当氧气受到限制时，在精氨酸上生长的PA0630菌株在稳定生长期会分泌瓜氨酸。这些观察结果表明，在有氧生长过程中，精氨酸并非仅通过双水解酶途径降解。
