Früh R, Watson J M, Haas D
Mol Gen Genet. 1983;191(2):334-7. doi: 10.1007/BF00334835.
The rec-102 mutation had pleiotropic effects in Pseudomonas aeruginosa: low recombinational proficiency in conjugation and transduction; high UV sensitivity; inability to induce pyocin R2 by mitomycin C; and increased susceptibility to mitomycin C and nalidixic acid. The rec-102 locus was mapped by R68.45-mediated conjugation in the 45 min region of the PAO chromosome, between argF and thr-9001. By selection for a marker in this region, rec-102 can be introduced into a P. aeruginosa strain of interest using an R68.45 rec-102 donor. The recombination-deficient strains constructed in this way were phenotypically similar to Escherichia coli recA mutants.
rec - 102突变在铜绿假单胞菌中具有多效性：在接合和转导中重组能力低；对紫外线高度敏感；无法被丝裂霉素C诱导产生绿脓菌素R2；对丝裂霉素C和萘啶酸的敏感性增加。rec - 102基因座通过R68.45介导的接合定位在PAO染色体45分钟区域，位于argF和thr - 9001之间。通过选择该区域的一个标记，可以使用R68.45 rec - 102供体将rec - 102引入感兴趣的铜绿假单胞菌菌株中。以这种方式构建的重组缺陷菌株在表型上与大肠杆菌recA突变体相似。
