Weber P, Held E, Uhlich E, Eigler J O
Kidney Int. 1975 May;7(5):331-41. doi: 10.1038/ki.1975.46.
Reaction constants of renin in juxtaglomerular apparatus and plasma renin activity after renal ischemia and hemorrhage. During and after total renal ischemia and acute hemorrhage, renin activity in plasma (PRA) and microdissected juxtaglomerular apparatus (JGA) of rabbits were investigated. In controls, the apparent Michaelis-Mentoen constant (MMC) of semipurified standard renin of rabbits was 1025 plus or minus 223 SD ng/ml. Plasma renin of normal rabbits showed similar values: 1062 plus or minus 138 SD ng/ml. Intrarenal JGA renin, however, showed a great scatter of MMC (920 to 4760 ng/ml) and a significantly higher mean value of 2572 plus or minus 1156 SD ng/ml (pis less than 0.001). After complete renal ischemia by clamping both renal arteries for a 90-min period, the following results wereobtained: 1) Sixty min after the beginning of ischemia, PRA decreased from 20.9 plus or minus 9.8 SD to 7.6 plus or minus 5.2 SD ng/ml-hr (P is less than 0.05) and increased to 103, 68 and 42 ng/ml-hr 10, 30 and 90 min after removal of the clamps, respectively (P is less than 0.05).
肾缺血和出血后肾小球旁器中肾素的反应常数及血浆肾素活性。在完全性肾缺血和急性出血期间及之后，对兔的血浆肾素活性（PRA）和经显微解剖的肾小球旁器（JGA）进行了研究。在对照组中，兔半纯化标准肾素的表观米氏常数（MMC）为1025±223标准差纳克/毫升。正常兔的血浆肾素显示出相似的值：1062±138标准差纳克/毫升。然而，肾内JGA肾素的MMC有很大的离散度（920至4760纳克/毫升），且平均值显著更高，为2572±1156标准差纳克/毫升（P<0.001）。通过夹闭双侧肾动脉90分钟造成完全性肾缺血后，得到以下结果：1）缺血开始60分钟后，PRA从20.9±9.8标准差降至7.6±5.2标准差纳克/毫升·小时（P<0.05），在解除夹闭后10、30和90分钟分别升至103、68和42纳克/毫升·小时（P<0.05）。
