Ichihara K, Yamakawa I, Kusunose E, Kusunose M
J Biochem. 1979 Jul;86(1):139-46.
The microsomes from rabbit intestinal mucosa which had been washed quickly and thoroughly with phenylmethylsulfonyl fluoride were found to catalyze the hydroxylation of fatty acids in the presence of NADPH and molecular oxygen. Myristic and palmitic acids were converted to the corresponding omega-and (omega-1)-hydroxy fatty acids, whereas lauric acid was converted only to 12-hydroxylauric acid, and capric acid, to 9-and 10-hydroxycapric acids together with an unknown polar acid. Among these fatty acids, both myristic and lauric acids appeared to be the most efficient substrates. The inhibition of the hydroxylation by SKF 525-A and carbon monoxide suggested that the activity depended upon cytochrome P-450. The specific activity of the fatty acid hydroxylation was almost constant along the small intestine, while the aminopyrine N-demethylation activity and the cytochrome P-450 content were highest at the proximal end of the intestine and progressively declined toward the caudal end. The cytochrome P-450 was solubilized from the intestinal microsomes and purified by 6-amino-n-hexyl Sepharose 4B chromatography. The partially purified cytochrome P-450 was active in fatty acid hydroxylation in combination with intestinal NADPH-cytochrome c reductase and phosphatidylcholine.
用苯甲基磺酰氟快速彻底洗涤过的兔肠黏膜微粒体，在存在烟酰胺腺嘌呤二核苷酸磷酸（NADPH）和分子氧的情况下，被发现可催化脂肪酸的羟基化反应。肉豆蔻酸和棕榈酸被转化为相应的ω-和（ω-1）-羟基脂肪酸，而月桂酸仅被转化为12-羟基月桂酸，癸酸则被转化为9-和10-羟基癸酸以及一种未知的极性酸。在这些脂肪酸中，肉豆蔻酸和月桂酸似乎是最有效的底物。SKF 525-A和一氧化碳对羟基化反应的抑制表明，该活性依赖于细胞色素P-450。脂肪酸羟基化的比活性在小肠中几乎恒定，而氨基比林N-脱甲基化活性和细胞色素P-450含量在小肠近端最高，并向尾端逐渐下降。细胞色素P-450从肠微粒体中溶解出来，并通过6-氨基正己基琼脂糖4B色谱法进行纯化。部分纯化的细胞色素P-450与肠NADPH-细胞色素c还原酶和磷脂酰胆碱结合时，在脂肪酸羟基化反应中具有活性。
