Nosaka K, Onozuka M, Kakazu N, Hibi S, Nishimura H, Nishino H, Abe T
Department of Chemistry, Kyoto Prefectural University of Medicine, Kita-ku, Kyoto 603-8334, Japan.
Biochim Biophys Acta. 2001 Jan 26;1517(2):293-7. doi: 10.1016/s0167-4781(00)00247-5.
A human thiamine pyrophosphokinase cDNA clone (hTPK1) was isolated and sequenced. When the intact hTPK1 open reading frame was expressed as a histidine-tag fusion protein in Escherichia coli, marked enzyme activity was detected in the bacterial cells. The hTPK1 mRNA was widely expressed in various human tissues at a very low level, and the mRNA content in cultured fibroblasts was unaffected by the thiamine concentration of the medium. The chromosome localization of the hTPK1 gene was assigned to 7q34.
分离并测序了人硫胺素焦磷酸激酶cDNA克隆（hTPK1）。当完整的hTPK1开放阅读框在大肠杆菌中作为组氨酸标签融合蛋白表达时，在细菌细胞中检测到显著的酶活性。hTPK1 mRNA在各种人体组织中广泛表达，但水平极低，并且培养的成纤维细胞中的mRNA含量不受培养基硫胺素浓度的影响。hTPK1基因的染色体定位被确定在7q34。
