Bord S, Horner A, Beavan S, Compston J
University of Cambridge School of Clinical Medicine, Addenbrooke's Hospital, United Kingdom.
J Clin Endocrinol Metab. 2001 May;86(5):2309-14. doi: 10.1210/jcem.86.5.7513.
Estrogen plays an essential role in the development and maintenance of the skeleton; its effects are mediated via interactions with two estrogen receptor (ER) subtypes, alpha and beta. The aim of this study was to establish the cellular distribution of ERalpha and ERbeta in neonatal human rib bone. ERalpha and ERbeta immunoreactivity was seen in proliferative and prehypertrophic chondrocytes in the growth plate, with lower levels of expression in the late hypertrophic zone. Different patterns of expression of the two ERs were seen in bone. In cortical bone, intense staining for ERalpha was observed in osteoblasts and osteocytes adjacent to the periosteal-forming surface and in osteoclasts on the opposing resorbing surface. In cancellous bone, ERbeta was strongly expressed in both osteoblasts and osteocytes, whereas only low expression of ERalpha was seen in these areas. Nuclear and cytoplasmic staining for ERbeta was apparent in osteoclasts. These observations demonstrate distinct patterns of expression for the two ER subtypes in developing human bone and indicate functions in both the growth plate and mineralized bone. In the latter, ERalpha is predominantly expressed in cortical bone, whereas ERbeta shows higher levels of expression in cancellous bone.
雌激素在骨骼的发育和维持中起着至关重要的作用；其作用是通过与两种雌激素受体（ER）亚型α和β相互作用介导的。本研究的目的是确定新生儿人肋骨中ERα和ERβ的细胞分布。在生长板的增殖性和前肥大性软骨细胞中可见ERα和ERβ免疫反应性，在肥大后期区域表达水平较低。在骨中观察到两种ER的不同表达模式。在皮质骨中，在与骨膜形成表面相邻的成骨细胞和骨细胞以及相对吸收表面的破骨细胞中观察到强烈的ERα染色。在松质骨中，ERβ在成骨细胞和骨细胞中均强烈表达，而在这些区域仅观察到ERα的低表达。破骨细胞中可见ERβ的核染色和胞质染色。这些观察结果表明，在发育中的人骨中，两种ER亚型具有不同的表达模式，并表明它们在生长板和矿化骨中均发挥作用。在后者中，ERα主要在皮质骨中表达，而ERβ在松质骨中表达水平较高。
