Wu J, Trogadis J, Bremner R
Vision Science Research Program, Cellular and Molecular Division, Toronto Western Research Institute, University Health Network, Toronto, Canada.
Mol Vis. 2001 Apr 26;7:101-6.
To determine whether p53 is required for the death of rod and cone photoreceptors in rd mice, a model of human retinitis pigmentosa, and/or for the natural degeneration of inner nuclear layer (INL) cells in the developing retina.
Rod photoreceptor and INL apoptosis was assessed by TUNEL staining of mouse sagittal sections from post natal day (P) 10, 13, 15, 17, and 20 day p53+/+ and p53-/- rd retinas. Cone photoreceptor survival was measured by counting the total number of peanut agglutinin (PNA) positive cells in eighty four 0.25 mm x 0.25 mm bins in each eye, distributed equally across the four quadrants of whole mount retinas from 3 month old p53+/+ and p53-/- rd retinas.
Both the kinetics of rod and INL cell death as well as the survival of cones were essentially unaffected by the absence of p53.
Despite established links with retinal apoptosis, p53 is not essential for rod or cone cell degeneration in the rd mouse or for the elimination of bipolar and Muller cells during late retinal development.
确定p53对于视网膜色素变性的模型rd小鼠中视杆和视锥光感受器的死亡，和/或对于发育中视网膜内核层（INL）细胞的自然退化是否是必需的。
通过对出生后第10、13、15、17和20天的p53+/+和p53-/- rd视网膜的小鼠矢状切片进行TUNEL染色，评估视杆光感受器和INL的凋亡情况。通过计数来自3个月大的p53+/+和p53-/- rd视网膜的全层视网膜四个象限中均匀分布的每只眼睛的84个0.25mm x 0.25mm区域内花生凝集素（PNA）阳性细胞的总数，来测量视锥光感受器的存活情况。
视杆和INL细胞死亡的动力学以及视锥细胞的存活基本上不受p53缺失的影响。
尽管已确定与视网膜凋亡有关，但p53对于rd小鼠中的视杆或视锥细胞退化或在视网膜发育后期消除双极细胞和穆勒细胞并非必不可少。
