Báez J L, Bolívar F, Gosset G
Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3. Cuernavaca, Morelos 62250, México.
Biotechnol Bioeng. 2001 Jun 20;73(6):530-5. doi: 10.1002/bit.1088.
The effect of inactivation of the glucose phosphotransferase transport system (PTS) on 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) productivity and yield from glucose in Escherichia coli is reported. Strains used in this study were the PTS(+) PB103 and its PTS(-) glucose(+) derivative NF9. Their aroB(-) derivatives PB103B and NF9B were constructed to allow accurate measurement of total carbon flow into the aromatic pathway. The measured specific rates of DAHP synthesis were 0.55 and 0.94 mmol/g-dcw. h and the DAHP molar yields from glucose were 0.43 and 0.71 mol/mol for the PTS(+) aroB(-)and the PTS(-) glucose(+) aroB(-)strains, respectively. For the latter strain, this value represents 83% of the maximum theoretical yield for DAHP synthesis from glucose.
报道了葡萄糖磷酸转移酶转运系统（PTS）失活对大肠杆菌中3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸（DAHP）产量以及葡萄糖生成DAHP产率的影响。本研究中使用的菌株为PTS(+) PB103及其PTS(-)葡萄糖(+)衍生物NF9。构建了它们的aroB(-)衍生物PB103B和NF9B，以便准确测量进入芳香族途径的总碳流量。PTS(+) aroB(-)和PTS(-)葡萄糖(+) aroB(-)菌株的DAHP合成比速率分别为0.55和0.94 mmol/g-dcw·h，葡萄糖生成DAHP的摩尔产率分别为0.43和0.71 mol/mol。对于后一种菌株，该值代表了从葡萄糖合成DAHP的最大理论产率的83%。
