卡氏肺孢子虫肺炎聚集病例的基因型。

Genotypes of clustered cases of Pneumocystis carinii pneumonia.

作者信息

Olsson M, Eriksson B M, Elvin K, Strandberg M, Wahlgren M

机构信息

Swedish Institute for Infectious Disease Control and Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden.

出版信息

Scand J Infect Dis. 2001;33(4):285-9. doi: 10.1080/003655401300077324.

DOI:10.1080/003655401300077324

PMID:11345221

Abstract

Reports of outbreaks of Pneumocystis carinii pneumonia (PCP) among human immunodeficiency virus-negative immunocompromised patients have suggested a person-to-person transmission of P. carinii. In this study, 17 bronchoalveolar lavage isolates from patients in 3 PCP outbreaks were genotyped, 2 in renal transplant recipients and 1 outbreak among patients with haematological disorders. Genotypes in the P. carinii sp. f. hominis (P. carinii f.sp. hominis) mt large subunit ribosomal RNA site 85 were detected by 2 methods: direct sequencing and 3 different allele-specific polymerase chain reaction assays. Although limited data on patient contacts were available, the detected P. c. hominis genotypes do not support person-to-person transmission as the predominant transmission route of P. carinii in humans.

摘要

在人类免疫缺陷病毒阴性的免疫功能低下患者中发生卡氏肺孢子虫肺炎（PCP）暴发的报告提示卡氏肺孢子虫可在人与人之间传播。在本研究中，对3起PCP暴发事件中患者的17株支气管肺泡灌洗分离株进行了基因分型，其中2起发生在肾移植受者中，1起发生在血液系统疾病患者中。采用两种方法检测卡氏肺孢子虫人型变种（P. carinii f.sp. hominis）线粒体大亚基核糖体RNA位点85的基因型：直接测序和3种不同的等位基因特异性聚合酶链反应检测法。尽管关于患者接触者的数据有限，但检测到的人型卡氏肺孢子虫基因型并不支持人与人之间传播是卡氏肺孢子虫在人类中的主要传播途径。