Ihira M, Yoshikawa T, Suzuki K, Ohashi M, Suga S, Asonuma K, Tanaka K, Asano Y
Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
Microbiol Immunol. 2001;45(3):225-32. doi: 10.1111/j.1348-0421.2001.tb02611.x.
Human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7) are closely related to each other. Interaction between the two viruses at the time of primary HHV-7 infection is suggested by in vivo and in vitro studies. However, interaction between the two viruses in organ transplant recipients has not been analyzed. We analyzed serially collected plasma samples obtained from 40 living related liver transplant recipients by serological assay (indirect immunofluorescence assay, IFA) and polymerase chain reaction (PCR). Significant increase or seroconversion of HHV-6 IgG and HHV-7 IgG antibody titers were observed in 45% and 58% of recipients respectively. Positive rate of IgM HHV-6 antibody increased up to 35% at 4 weeks after transplantation. However, no remarkable peak in the positive rate of HHV-7 IgM antibody was demonstrated. HHV-6 and HHV-7 DNA were detected in plasma in 15 (38%) and 16 (40%) of the 40 recipients respectively. HHV-6 DNA was detected in 10 (26%) of the 38 recipients at 2 weeks after transplantation. The positive rate of the virus genome in plasma gradually decreased after that time. HHV-7 DNA was detected in 5 (14%) of the 37 recipients at 2 weeks after transplantation; no obvious peak in the positive rate of HHV-7 DNA was demonstrated. Antibody responses involving both HHV-6 and HHV-7, including either a significant increase in IgG antibody titers or positive identification of IgM antibody were observed in 17 (43%) of the 40 recipients. Thirteen out of the 17 recipients demonstrated concurrent antibody response against both viruses. HHV-7 antibody response preceded the HHV-6 antibody response in 2 of the remaining 4 recipients, whereas the opposite was true in the other 2 recipients. Both HHV-6 and HHV-7 DNA were detected in 7 (18%) of the 40 recipients. In 4 of those 7 recipients, DNA from both viruses was concurrently detected, 3 of whom had HHV-7 DNA repeatedly detected after first detection of the virus DNA. The detection of HHV-7 DNA preceded the detection of HHV-6 DNA in 2 recipients, whereas HHV-6 DNA appeared first in 1 recipient.
人类疱疹病毒6型（HHV-6）和人类疱疹病毒7型（HHV-7）彼此密切相关。体内和体外研究表明，在原发性HHV-7感染时，这两种病毒之间存在相互作用。然而，尚未分析器官移植受者中这两种病毒之间的相互作用。我们通过血清学检测（间接免疫荧光法，IFA）和聚合酶链反应（PCR）分析了从40名活体亲属肝移植受者中连续采集的血浆样本。分别在45%和58%的受者中观察到HHV-6 IgG和HHV-7 IgG抗体滴度显著升高或血清转化。移植后4周时，HHV-6 IgM抗体阳性率升至35%。然而，未观察到HHV-7 IgM抗体阳性率出现明显峰值。在40名受者中，分别有15名（38%）和16名（40%）的血浆中检测到HHV-6和HHV-7 DNA。在移植后2周时，38名受者中有10名（26%）检测到HHV-6 DNA。此后，血浆中病毒基因组的阳性率逐渐下降。在移植后2周时，37名受者中有5名（14%）检测到HHV-7 DNA；未观察到HHV-7 DNA阳性率出现明显峰值。在40名受者中，有17名（43%）观察到涉及HHV-6和HHV-7的抗体反应，包括IgG抗体滴度显著升高或IgM抗体阳性鉴定。17名受者中有13名表现出对两种病毒的同时抗体反应。其余4名受者中有2名的HHV-7抗体反应先于HHV-6抗体反应，而另外2名受者则相反。40名受者中有7名（18%）同时检测到HHV-6和HHV-7 DNA。在这7名受者中的4名中，同时检测到两种病毒的DNA，其中3名在首次检测到病毒DNA后多次检测到HHV-7 DNA。2名受者中HHV-7 DNA的检测先于HHV-6 DNA，而1名受者中HHV-6 DNA最先出现。
