Czech E, Kneifel W, Kopp B
Department of Dairy Research and Bacteriology, University of Agricultural Sciences, Vienna, Austria.
Planta Med. 2001 Apr;67(3):263-9. doi: 10.1055/s-2001-12007.
One hundred and thirty-eight medicinal herbal drugs obtained from different suppliers were examined for microbial contaminants and for the detectability of pathogenic microorganisms. For this purpose, several microbiological standard parameters (total aerobic mesophilic count, enterobacteria, coliforms, aerobic sporeformers, yeasts and moulds, enterococci, lactobacilli, pseudomonades and aeromades) and selective methods for the detection of indicator microorganisms and pathogens (E. coli, enterohaemorrhagic E. coli [EHEC], Salmonella, Campylobacter jejuni, Pseudomonas aeruginosa, Bacillus cereus, Clostridium perfringens, Listeria, coagulase-positive staphylococci, Candida albicans, potentially aflatoxigenic moulds) were applied. The microbial load of the samples varied considerably. While none of the samples contained EHEC, Salmonellae, Pseudomonas aeruginosa, Listeriae, Staphylococcus aureus or Candida albicans, four samples were E. coli positive, two samples were presumptively Campylobacter jejuni positive and nine herbal drugs contained a potentially aflatoxigenic mould flora. Further details regarding different viable count classes as well as preparation techniques are discussed.
对从不同供应商处获得的138种药草进行了微生物污染物及致病微生物可检测性检查。为此，采用了多个微生物学标准参数（需氧嗜温菌总数、肠杆菌、大肠菌群、需氧芽孢杆菌、酵母和霉菌、肠球菌、乳酸菌、假单胞菌和气单胞菌）以及检测指示微生物和病原体（大肠杆菌、肠出血性大肠杆菌[EHEC]、沙门氏菌、空肠弯曲菌、铜绿假单胞菌、蜡样芽孢杆菌、产气荚膜梭菌、李斯特菌、凝固酶阳性葡萄球菌、白色念珠菌、潜在产黄曲霉毒素霉菌）的选择性方法。样品的微生物负荷差异很大。虽然没有样品含有EHEC、沙门氏菌、铜绿假单胞菌、李斯特菌、金黄色葡萄球菌或白色念珠菌，但有4个样品大肠杆菌呈阳性，2个样品疑似空肠弯曲菌呈阳性，9种药草含有潜在产黄曲霉毒素的霉菌菌群。文中还讨论了不同活菌计数类别以及制备技术的更多细节。
