Abba Danladi, Inabo Helen I, Yakubu Sabo E, Olonitola Olayeni S
Department of Microbiology, Ahmadu Bello University, Zaria, Nigeria.
Afr J Tradit Complement Altern Med. 2008 Oct 25;6(1):70-7. doi: 10.4314/ajtcam.v6i1.57076.
The study aimed to evaluate the bacterial contamination of powdered herbal medicinal preparations sourced from identified herbal retail outlets in different parts of Kaduna metropolis. The assessments of the contamination of the herbal products were carried out using standard procedures: total aerobic bacterial plate count, measurement of some physical parameters, isolation and characterization of selected bacterial pathogens etc. The results showed that out of a total of 150, 70 (46.67%) herbal remedies were contaminated with Salmonella typhi, twenty nine (19.33%) with Shigella spp. Eighty eight (58.67%) and 98 (65.33%) were contaminated with Escherichia coli and Staphylococcus aureus, respectively. The total aerobic plate count results showed that the highest average count of > 5x10(7) cfu/g was found in 89 (59.33%) of the preparations, while average plate count of < or = 5x10(7) cfu/g was found in 42 (28%) and no bacterial count was obtained in 19 (12.67%) of the preparations. Correlation was positive (P = 0.01; r = +0.109) between the physical parameters tested and the bacterial load. Antibacterial activities result of some common antibiotics showed that all the antibiotics had activities on the test bacterial isolates at various minimum inhibitory concentrations. Most traditionally prepared herbal medications in Kaduna state are likely to be contaminated with a wide variety of potentially pathogenic bacteria. The quality assurance of these products should be thoroughly enforced and monitored in the production and distribution of herbal preparations.
该研究旨在评估从卡杜纳市不同地区特定草药零售店采购的草药粉剂制剂的细菌污染情况。采用标准程序对草药产品的污染情况进行评估：需氧菌总数平板计数、一些物理参数的测量、选定细菌病原体的分离和鉴定等。结果显示，在总共150种草药制剂中，70种（46.67%）被伤寒沙门氏菌污染，29种（19.33%）被志贺氏菌属污染。分别有88种（58.67%）和98种（65.33%）被大肠杆菌和金黄色葡萄球菌污染。需氧菌总数平板计数结果表明，89种（59.33%）制剂的平均计数最高，>5×10⁷ cfu/g，而42种（28%）制剂的平均平板计数<或 = 5×10⁷ cfu/g，19种（12.67%）制剂未获得细菌计数。所测试的物理参数与细菌载量之间存在正相关（P = 0.01；r = +0.109）。一些常见抗生素的抗菌活性结果表明，所有抗生素在不同的最低抑菌浓度下对测试细菌分离株均有活性。卡杜纳州大多数传统制备的草药药物可能被多种潜在致病细菌污染。在草药制剂的生产和分销过程中，应全面加强并监测这些产品的质量保证。
