Yun J, Oh S R, Lee H K, Chang I M, Takatsu K, Yun Y P, Min K R, Kim Y
Planta Med. 2001 Apr;67(3):274-6. doi: 10.1055/s-2001-12002.
Effects of genistein analogs on oxygen radical production have been analyzed in human neutrophils, human monocytes or murine macrophages Raw264.7 stimulated with unopsonized zymosan by lucigenin- and luminol-enhanced chemiluminescence assays. Genistein exhibited IC50 values of 10.7-11.5 microM on the oxygen radical production in human neutrophils, 10.9-11.0 microM in human monocytes, and 14.8-27.3 microM in Raw264.7 cells. Orobol, a genistein analog with an additional hydroxy group at the 3' position, exhibited IC50 values of 3.0-3.3 microM on the oxygen radical production in human neutrophils, 2.8-3.1 microM in human monocytes, and 1.5-3.9 microM in Raw264.7 cells. Genistin and sophoricoside are genistein glycosides with a glucose moiety at 7 or 4' position, respectively. The genistein glycosides exhibited 23-37% inhibitory effects at 100 microM on the oxygen radical production.
通过光泽精和鲁米诺增强化学发光分析，已在未调理酵母聚糖刺激的人中性粒细胞、人单核细胞或小鼠巨噬细胞Raw264.7中分析了染料木黄酮类似物对氧自由基产生的影响。染料木黄酮对人中性粒细胞氧自由基产生的IC50值为10.7 - 11.5微摩尔，对人单核细胞为10.9 - 11.0微摩尔，对Raw264.7细胞为14.8 - 27.3微摩尔。奥罗波尔是一种在3'位带有额外羟基的染料木黄酮类似物，对人中性粒细胞氧自由基产生的IC50值为3.0 - 3.3微摩尔，对人单核细胞为2.8 - 3.1微摩尔，对Raw264.7细胞为1.5 - 3.9微摩尔。染料木苷和槐角苷分别是在7位或4'位带有葡萄糖部分的染料木黄酮糖苷。染料木黄酮糖苷在100微摩尔时对氧自由基产生表现出23 - 37%的抑制作用。
