Gallori S, Bilia A R, Mulinacci N, Bicchi C, Rubiolo P, Vincieri F F
Planta Med. 2001 Apr;67(3):290-2. doi: 10.1055/s-2001-12001.
The volatile fraction of Tambourissa leptophylla fruit skin was extracted by petrol ether, purified by adsorption chromatography (LPC) and analysed by gas chromatographic-spectroscopic methods. 27 non-oxygenated terpene hydrocarbons and 10 oxygenated derivatives were identified. The most abundant components were: limonene (24.0%), cis-alpha-bergamotene (23.2%), delta-3-carene (8.2%), alpha-curcumene (6.0%), trans-alpha-bergamotene (5.1%), alpha-copaene (4.1%), alpha-pinene (4.0%), p-cymene (4.0%) and bicyclogermacrene (3.3%). The crude volatile fraction was tested in vitro against Cladosporium cucumerinum in direct bioautography on TLC plates on the basis of the antifungal use of fruit skin. Activity of petrol ether extract against this micro-organism was demonstrated.
用石油醚提取了细叶坦布黎萨果皮的挥发性成分，通过吸附色谱法（LPC）进行纯化，并采用气相色谱-光谱法进行分析。鉴定出27种非氧化萜类碳氢化合物和10种氧化衍生物。含量最丰富的成分有：柠檬烯（24.0%）、顺式-α-佛手柑烯（23.2%）、δ-3-蒈烯（8.2%）、α-姜黄烯（6.0%）、反式-α-佛手柑烯（5.1%）、α-可巴烯（4.1%）、α-蒎烯（4.0%）、对伞花烃（4.0%）和双环大根香叶烯（3.3%）。基于果皮的抗真菌用途，在TLC板上通过直接生物自显影法对粗挥发性成分进行了体外抗黄瓜枝孢菌测试。结果表明石油醚提取物对这种微生物具有活性。
