[12-羟基-9(Z)-十二碳烯酸对豌豆根生长和细胞分裂的影响]

[Effect of 12-hydroxy-9(Z)-dodecenoic acid on growth and cell division in pea roots].

作者信息

Ivanov A B, Iarin A Iu, Grechkin A N, Tarchevski I A

机构信息

Institute of Biochemistry and Biophysics, Kazan Research Centre, Russian Academy of Sciences, Kazan.

出版信息

Tsitologiia. 2001;43(2):166-71.

PMID:11347472

Abstract

It is known that potential bioregulators may be present among lipoxygenase oxidation products. A possibility of mitotic cycle regulation by 12-hydroxy-9(Z)-dodecenic acid (12-HDA) and also its influence on the growing function (seed germination, root and epicotyl growth) have been studied. It has been determined that 12-HDA activity is directed to the strengthening of growing function which allowed to suppose that oxylipin is capable of regulating cell division. 12-HDA participation in the mitotic cycle regulation were determined by the originally developed test system using simultaneously light microscopy. The concentration and temporal dependencies of cell division were studied under the influence of 12-HDA. The raise of mitosis (up to 17.5 times) has been registered in comparison with the control variant by the least concentration of 12-HDA (10(-9) M) up to the 4th h of influence, confirming oxypilin participation in mitotic cycle regulation.

摘要

已知潜在的生物调节剂可能存在于脂氧合酶氧化产物中。研究了12-羟基-9(Z)-十二碳烯酸（12-HDA）对有丝分裂周期的调节作用及其对生长功能（种子萌发、根和上胚轴生长）的影响。已确定12-HDA的活性旨在增强生长功能，这使得人们推测氧脂能够调节细胞分裂。12-HDA参与有丝分裂周期调节是通过最初开发的同时使用光学显微镜的测试系统确定的。研究了在12-HDA影响下细胞分裂的浓度和时间依赖性。与对照变体相比，在12-HDA最低浓度（10(-9) M）作用至第4小时时，有丝分裂增加（高达17.5倍），证实氧脂参与有丝分裂周期调节。