Shirota K, Katoh H, Murase T, Ito T, Otsuki K
Poultry Products Quality Control Co, Ltd, Nihonmats, Fukushima, Japan.
J Food Prot. 2001 May;64(5):734-7. doi: 10.4315/0362-028x-64.5.734.
In order to investigate contamination of chicken farms with Salmonella, feed and eggs were sampled from 16 commercial layer farms in eastern Japan between 1993 and 1998 and cultured for salmonellae. Salmonella enterica subsp. enterica isolates belonging to 19 serovars were obtained from the feed. Six of the 19 serotypes, including Salmonella serovar Enteritidis, were observed in isolates recovered from the eggs. Salmonella serovar Enteritidis strains obtained from a feed sample and egg contents in a layer farm showed pulsed-field gel electrophoresis patterns that were genetically related and belonged to a single phage type, suggesting that the contamination of the farms was linked to the occurrence of salmonellae in feed.
为调查养鸡场沙门氏菌污染情况，于1993年至1998年期间从日本东部16个商业化蛋鸡养殖场采集饲料和鸡蛋样本，并培养沙门氏菌。从饲料中分离出属于19个血清型的肠炎沙门氏菌亚种。从鸡蛋中分离出的菌株中有19种血清型中的6种，包括肠炎沙门氏菌血清型。从一个蛋鸡养殖场的饲料样本和鸡蛋内容物中获得的肠炎沙门氏菌菌株显示出脉冲场凝胶电泳图谱，这些图谱在遗传上相关且属于单一噬菌体类型，这表明养殖场的污染与饲料中沙门氏菌的出现有关。
