Li R, Faraco J H, Lin L, Lin X, Hinton L, Rogers W, Lowe J K, Ostrander E A, Mignot E
Room P-114, Stanford Center for Narcolepsy Research, 1201 Welch Road, Stanford, California 94305-5485, USA.
Genomics. 2001 May 1;73(3):299-315. doi: 10.1006/geno.2000.6487.
The positional cloning of the hypocretin receptor 2, the gene for autosomal recessive canine narcolepsy, has led to the development of a physical map spanning a large portion of canine chromosome 12 (CFA12), in a region corresponding to human chromosome 6p12-q13. More than 40 expressed sequence tags (ESTs) were used in homology search experiments, together with chromosome walking, to build both physical and radiation hybrid maps of the CFA12 13-21 region. The resulting map of bacterial artificial chromosome ends, ESTs, and microsatellite markers represents the longest continuous high-density map of the dog genome reported to date. These data further establish the dog as a system for studying disease genes of interest to human populations and highlight feasible approaches for positional cloning of disease genes in organisms where genomic resources are limited.
