Paux Etienne, Sourdille Pierre, Salse Jérôme, Saintenac Cyrille, Choulet Frédéric, Leroy Philippe, Korol Abraham, Michalak Monika, Kianian Shahryar, Spielmeyer Wolfgang, Lagudah Evans, Somers Daryl, Kilian Andrzej, Alaux Michael, Vautrin Sonia, Bergès Hélène, Eversole Kellye, Appels Rudi, Safar Jan, Simkova Hana, Dolezel Jaroslav, Bernard Michel, Feuillet Catherine
Institut National de la Recherche Agronomique, Université Blaise Pascal (INRA-UBP), UMR 1095, Genetics Diversity and Ecophysiology of Cereals, Clermont-Ferrand, France.
Science. 2008 Oct 3;322(5898):101-4. doi: 10.1126/science.1161847.
As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence-based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17-billion-base-pair hexaploid bread wheat genome (2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome (BAC)-based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome-specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach.
作为全球35%人口的主食，小麦是最重要的作物种类之一。到目前为止，仍缺乏用于加速小麦改良的基于序列的工具。作为对170亿碱基对的六倍体面包小麦基因组（2n = 6x = 42条染色体）进行测序的国际努力的一部分，我们构建了基于细菌人工染色体（BAC）的最大染色体3B的整合物理图谱，该染色体本身就有995兆碱基。利用一个染色体特异性BAC文库将该染色体的82%组装成1036个重叠群，并用1443个分子标记进行定位，为遗传和基因组研究提供了一个重要资源。该物理图谱为其余小麦染色体建立了一个模板，并证明了采用基于染色体的方法构建大型、复杂多倍体基因组物理图谱的可行性。
