少即是多——使用重新设计的引物缩短STR扩增子长度
Less is more--length reduction of STR amplicons using redesigned primers.
作者信息
Wiegand P, Kleiber M
机构信息
Institute of Pathology and Legal Medicine, Department of Legal Medicine, University of Ulm, Prittwitzstrasse 6, 89075 Ulm, Germany.
出版信息
Int J Legal Med. 2001;114(4-5):285-7. doi: 10.1007/s004140000162.
PCR primers closely flanking the repeat region were redesigned to reduce the amplicon length of the selected STRs down to approximately 100 bp for the shorter alleles (loci HumTH01, D10S2325, DYS19 and DYS391). Highly degraded DNA (e.g. formalin-fixed tissue) and very low amounts of DNA could be more successfully typed using the new redesigned primers compared to the established sequences generating longer amplicons.
紧邻重复区域的聚合酶链反应（PCR）引物被重新设计，以便将所选短串联重复序列（STR）的扩增子长度缩短至较短等位基因（基因座HumTH01、D10S2325、DYS19和DYS391）约100碱基对。与产生较长扩增子的既定序列相比，使用新的重新设计引物，高度降解的DNA（如福尔马林固定组织）和极少量的DNA能够更成功地进行分型。
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