Turrina Stefania, Atzei Renzo, Filippini Giulia, De Leo Domenico
Department of Medicine and Public Health, Institute of Legal Medicine, Forensic Genetic Laboratory, University of Verona, 37134 Verona, Italy.
J Forensic Leg Med. 2008 Jan;15(1):27-31. doi: 10.1016/j.jcfm.2006.10.012. Epub 2007 Jan 18.
Three new mini-STR primer sets are suggested for three conventional STRs, CSF1P0, D8S1179 and D13S317, included in multiplex PCR kits commercially available and commonly used for DNA typing in forensic applications. The primer pairs for the three loci were redesigned in order to reduce or eliminate the flanking regions of the polymorphism obtaining amplification products, which have dimensions less than 120bp in size. A comparison of results for typing carried out with the newly designed primers on DNA extracted from 100 blood samples provided by healthy donors, previously typed with conventional STRs, showed no genotype difference underlining their precision and reproducibility. The forensic usefulness of the new mini-STR primers was evaluated on highly degraded DNA from casework samples (e.g. archival post-mortem Bouin's fluid-fixed paraffin-embedded tissue specimens) for which commercial STR kit had proven inefficient.
针对商业上可获得且常用于法医应用中DNA分型的多重PCR试剂盒中包含的三个传统STR（CSF1P0、D8S1179和D13S317），建议使用三种新的微型STR引物组。重新设计了这三个基因座的引物对，以减少或消除多态性的侧翼区域，从而获得大小小于120bp的扩增产物。对从100名健康献血者提供的血液样本中提取的DNA进行分型，这些样本之前已用传统STR进行过分型，结果显示，使用新设计的引物进行分型的结果与之前一致，没有基因型差异，突出了它们的准确性和可重复性。在来自实际案件样本（如存档的死后经布因氏液固定的石蜡包埋组织标本）的高度降解DNA上评估了新微型STR引物的法医实用性，而商业STR试剂盒已证明对这些样本无效。
