人类休止期毛发的STR分型——一种新方法。
STR typing of human telogen hairs--a new approach.
作者信息
Hellmann A, Rohleder U, Schmitter H, Wittig M
机构信息
Bundeskriminalamt, Kriminaltechnisches Institut, Thaerstrasse 11, 65193 Wiesbaden, Germany.
出版信息
Int J Legal Med. 2001;114(4-5):269-73. doi: 10.1007/s004140000175.
A new approach to short tandem repeat (STR) typing of DNA extracted from telogen shed hairs is presented. Newly designed primer pairs with annealing positions close to the repeat units of the STR loci HUMFES, HUMTH01 and HUMTPOX were used for amplification. The typing results were compared to those obtained by the commonly used primer pairs by means of success rates. The primer pairs capable of producing very short amplicons (< 106 bp with HUMFES, < 86 bp with HUMTH01 and < 87 bp with HUMTPOX) described in this study significantly increased the success rates when typing telogen hairs.
本文提出了一种对从休止期脱落毛发中提取的DNA进行短串联重复序列（STR）分型的新方法。使用新设计的引物对进行扩增，这些引物对的退火位置靠近STR基因座HUMFES、HUMTH01和HUMTPOX的重复单元。通过成功率将分型结果与常用引物对获得的结果进行比较。本研究中描述的能够产生非常短扩增子的引物对（HUMFES的<106 bp、HUMTH01的<86 bp和HUMTPOX的<87 bp）在对休止期毛发进行分型时显著提高了成功率。
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