Osada M, Inaba R, Shinohara H, Hagiwara M, Nakamura M, Ikawa Y
Human Gene Sciences Center, Medical Research Institute, Tokyo Medical and Dental University, Yushima 1-5-45, Tokyo, Bunkyo-ku, 113-8510, Japan.
Biochem Biophys Res Commun. 2001 May 25;283(5):1135-41. doi: 10.1006/bbrc.2001.4905.
The amino terminal of human P51/TAp63, a P53 homologue, possesses a transactivation domain involved in the activation of its target genes by binding to DNA elements responsive to the p53 protein family. Using a series of amino terminal deletions, the transactivation domain was mapped between amino acid residues 50 to 69. This domain also regulates protein stability in a proteasome-dependent manner, and Ser51 and Ser68 were found to be essential for this stability. Our results suggest that P51 activity is greatly affected by protein stability.
人P51/TAp63是一种P53同源物，其氨基末端拥有一个反式激活结构域，该结构域通过与对p53蛋白家族有反应的DNA元件结合来激活其靶基因。通过一系列氨基末端缺失实验，反式激活结构域被定位在氨基酸残基50至69之间。该结构域还以蛋白酶体依赖性方式调节蛋白质稳定性，并且发现Ser51和Ser68对于这种稳定性至关重要。我们的结果表明，P51活性受蛋白质稳定性的极大影响。
