Tello M, Seelenfreund D, Lobos S, Gaskell J, Cullen D, Vicuña R
Departamento de Bioquímica y Biología Molecular , Faculdade de Ciencias Químicas y Farmacéuticas, Universidade de Chile, Santiago.
FEMS Microbiol Lett. 2001 May 15;199(1):91-6. doi: 10.1111/j.1574-6968.2001.tb10656.x.
Genetic analyses of the lignin-degrading fungus Ceriporiopsis subvermispora is complicated by a dikaryotic nuclear condition and the absence of spore forms. Previous investigations had identified a family of closely related sequences encoding manganese peroxidase (MnP), but the relationship between genes and allelic variants could not be experimentally established. Addressing this issue, homokaryotic derivatives of C. subvermipora strain FP105752 were isolated from regenerated protoplasts. Designated CsA and CsB, their homokaryotic nature was established by polymerase chain reaction amplification and sequence analysis of the allelic variants of three MnP genes. Isoelectrofocusing revealed fewer MnP isoenzymes in filtrates of homokaryon cultures relative to the parental strain. The homokaryotic strains will simplify genetic analyses, particularly the identification of new genes.
