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黄孢原毛平革菌和亚侧耳栓菌中的类脂基因,这两种白腐真菌没有可检测到的木质素过氧化物酶活性。

Lip-like genes in Phanerochaete sordida and Ceriporiopsis subvermispora, white rot fungi with no detectable lignin peroxidase activity.

作者信息

Rajakumar S, Gaskell J, Cullen D, Lobos S, Karahanian E, Vicuna R

机构信息

USDA Forest Products Laboratory, Madison, Wisconsin 53705, USA.

出版信息

Appl Environ Microbiol. 1996 Jul;62(7):2660-3. doi: 10.1128/aem.62.7.2660-2663.1996.

DOI:10.1128/aem.62.7.2660-2663.1996
PMID:8779605
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC168048/
Abstract

Lignin peroxidase-like genes were PCR amplified from Phanerochaete sordida and Ceriporiopsis subvermispora, fungi lacking lignin peroxidase (LiP) activity. Amplification products were highly similar to previously described LiP genes. Using reverse transcription-coupled PCR a LiP-like cDNA clone was amplified from P. sordida RNA. In contrast, no evidence was obtained for transcription of C. subvermispora LiP genes.

摘要

从缺乏木质素过氧化物酶(LiP)活性的真菌——黄孢原毛平革菌和亚侧耳中通过聚合酶链式反应(PCR)扩增出类木质素过氧化物酶基因。扩增产物与先前描述的LiP基因高度相似。利用逆转录偶联PCR从黄孢原毛平革菌RNA中扩增出一个类LiP cDNA克隆。相比之下,未获得亚侧耳LiP基因转录的证据。

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