Rajakumar S, Gaskell J, Cullen D, Lobos S, Karahanian E, Vicuna R
USDA Forest Products Laboratory, Madison, Wisconsin 53705, USA.
Appl Environ Microbiol. 1996 Jul;62(7):2660-3. doi: 10.1128/aem.62.7.2660-2663.1996.
Lignin peroxidase-like genes were PCR amplified from Phanerochaete sordida and Ceriporiopsis subvermispora, fungi lacking lignin peroxidase (LiP) activity. Amplification products were highly similar to previously described LiP genes. Using reverse transcription-coupled PCR a LiP-like cDNA clone was amplified from P. sordida RNA. In contrast, no evidence was obtained for transcription of C. subvermispora LiP genes.
从缺乏木质素过氧化物酶(LiP)活性的真菌——黄孢原毛平革菌和亚侧耳中通过聚合酶链式反应(PCR)扩增出类木质素过氧化物酶基因。扩增产物与先前描述的LiP基因高度相似。利用逆转录偶联PCR从黄孢原毛平革菌RNA中扩增出一个类LiP cDNA克隆。相比之下,未获得亚侧耳LiP基因转录的证据。