小鼠生殖细胞在精原干细胞移植后完成精子发生不需要功能性雄激素受体。

Murine germ cells do not require functional androgen receptors to complete spermatogenesis following spermatogonial stem cell transplantation.

作者信息

Johnston D S, Russell L D, Friel P J, Griswold M D

机构信息

School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman 99164, USA.

出版信息

Endocrinology. 2001 Jun;142(6):2405-8. doi: 10.1210/endo.142.6.8317.

DOI:10.1210/endo.142.6.8317

PMID:11356688

Abstract

The spermatogonial stem cell transplantation technique was employed to determine if murine germ cells require functional androgen receptors to complete qualitatively normal spermatogenesis. Testicular cells from testicular feminized mice were injected into the seminiferous tubules of azoospermic mice expressing functional androgen receptors. Recipient testes were analyzed between 110 and 200 days following transplantation. Multiple colonies of complete and qualitatively normal donor-derived spermatogenesis were seen within the seminiferous tubules of each recipient testis, demonstrating that murine germ cells do not require functional androgen receptors to complete spermatogenesis.

摘要

采用精原干细胞移植技术来确定小鼠生殖细胞是否需要功能性雄激素受体以完成质量正常的精子发生过程。将睾丸雌性化小鼠的睾丸细胞注射到表达功能性雄激素受体的无精子症小鼠的生精小管中。在移植后110至200天之间对受体睾丸进行分析。在每个受体睾丸的生精小管内可见多个完整且质量正常的供体来源精子发生的克隆，这表明小鼠生殖细胞不需要功能性雄激素受体来完成精子发生。