Brinster R L, Zimmermann J W
Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.
Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11298-302. doi: 10.1073/pnas.91.24.11298.
In the adult male, a population of diploid stem-cell spermatogonia continuously undergoes self-renewal and produces progeny cells, which initiate the complex process of cellular differentiation that results in mature spermatozoa. We report here that stem cells isolated from testes of donor male mice will repopulate sterile testes when injected into seminiferous tubules. Donor cell spermatogenesis in recipient testes showed normal morpholigical characteristics and produced mature spermatozoa. This methodology, besides opening new avenues of basic research into spermatogenesis and stem-cell self-renewal, may prove useful as a tool for biomedical science and biotechnology.
在成年雄性中，二倍体干细胞精原细胞群体持续进行自我更新并产生子代细胞，这些子代细胞启动导致成熟精子的复杂细胞分化过程。我们在此报告，从供体雄性小鼠睾丸分离出的干细胞注入生精小管后，将重新填充不育睾丸。受体睾丸中的供体细胞精子发生表现出正常的形态特征并产生成熟精子。这种方法除了为精子发生和干细胞自我更新的基础研究开辟新途径外，还可能被证明是生物医学科学和生物技术的有用工具。
