利用荧光磷脂报告基因对消化生理进行遗传分析。

Genetic analysis of digestive physiology using fluorescent phospholipid reporters.

作者信息

Farber S A, Pack M, Ho S Y, Johnson I D, Wagner D S, Dosch R, Mullins M C, Hendrickson H S, Hendrickson E K, Halpern M E

机构信息

Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA.

出版信息

Science. 2001 May 18;292(5520):1385-8. doi: 10.1126/science.1060418.

DOI:10.1126/science.1060418

PMID:11359013

Abstract

Zebrafish are a valuable model for mammalian lipid metabolism; larvae process lipids similarly through the intestine and hepatobiliary system and respond to drugs that block cholesterol synthesis in humans. After ingestion of fluorescently quenched phospholipids, endogenous lipase activity and rapid transport of cleavage products results in intense gall bladder fluorescence. Genetic screening identifies zebrafish mutants, such as fat free, that show normal digestive organ morphology but severely reduced phospholipid and cholesterol processing. Thus, fluorescent lipids provide a sensitive readout of lipid metabolism and are a powerful tool for identifying genes that mediate vertebrate digestive physiology.

摘要

斑马鱼是研究哺乳动物脂质代谢的重要模型；幼虫通过肠道和肝胆系统以类似方式处理脂质，并对阻断人类胆固醇合成的药物产生反应。摄入荧光淬灭的磷脂后，内源性脂肪酶活性和裂解产物的快速转运导致胆囊发出强烈荧光。基因筛选可鉴定出斑马鱼突变体，如无脂肪突变体，其消化器官形态正常，但磷脂和胆固醇处理能力严重降低。因此，荧光脂质可灵敏反映脂质代谢情况，是鉴定介导脊椎动物消化生理的基因的有力工具。