中国阻塞性睡眠呼吸暂停低通气综合征患者血管紧张素I转换酶基因多态性

[Angiotension I converting enzyme gene polymorphism in Chinese patients with obstructive sleep apnea syndrome].

作者信息

Xiao Y, Huang X, Qiu C

机构信息

Sleep Disorders Center, PUMC Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100730.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 1998 Aug;21(8):489-91.

PMID:11360521

Abstract

OBJECTIVE

To investigate the relationship of an insertion/deletion (I/D) polymorphism of the angiotension-converting enzyme (ACE) gene to obstructive sleep apnea syndrome (OSAS).

METHOD

Genomic DNA was extracted from blood samples and amplified by polymerase chain reaction (PCR). PCR primers were flanking the polymorphic region in intron 16 of the ACE gene.

RESULT

The distribution of the DD, ID, and II ACE genotypes was 16%, 52%, and 32% in the control subjects and 0%, 56%, and 44% in OSAS patients, respectively. The estimated frequencies of the insertion allele and the deletion allele were 58%, 42% in the control subjects and 72%, 28% in OSAS patients, respectively. The differences were statistically significant(P < 0.05). The OSAS patients with I/I genotype had significantly longer apnea time (P < 0.05), lower minimum SaO2(P < 0.05) and more severe AHI (P < 0.05) than did the OSAS patients with I/D genotype.

CONCLUSION

These results indicate that the II genotype and I allele might be a risk factor for OSAS in Chinese.

摘要

目的

探讨血管紧张素转换酶（ACE）基因插入/缺失（I/D）多态性与阻塞性睡眠呼吸暂停综合征（OSAS）的关系。

方法

从血样中提取基因组DNA，采用聚合酶链反应（PCR）进行扩增。PCR引物位于ACE基因第16内含子多态性区域两侧。

结果

对照组中DD、ID和II ACE基因型的分布分别为16%、52%和32%，OSAS患者中分别为0%、56%和44%。插入等位基因和缺失等位基因的估计频率在对照组中分别为58%、42%，在OSAS患者中分别为72%、28%。差异具有统计学意义（P<0.05）。与I/D基因型的OSAS患者相比，I/I基因型的OSAS患者呼吸暂停时间明显更长（P<0.05），最低血氧饱和度更低（P<0.05），呼吸暂停低通气指数（AHI）更严重（P<0.05）。