Sun L, Zheng Y, Wang C
Second Affiliated Hospital, Henan Medical University, Zhengzhou 450003.
Zhonghua Fu Chan Ke Za Zhi. 1999 Sep;34(9):555-7.
To investigate the effects of mifepristone on cell proliferation and hCG secretion of human choriocarcinoma cell line JAR and the cytotoxic effects of mifepristone on this cell line.
The cytotoxic effects of four different concentration of mifepristone (1,5,10,20 mumol/L) were assessed with methyl thiazolyl tetrazolium (MTT) colorimetric assay. The expression of nuclear proliferative antigen Ki-67 and the amount of hCG secreation in JAR cells were detected with avidin-biotin complex method, immuno-histochemical method and enzyme-linked immunosorbent assay (ELISA) respectively.
There were dose-dependent cytotoxic effects on JAR cells in four different concentrations of mifepristone. This effect plateaued at 20 mumol/L. When the JAR cells were exposed to 5 mumol/L mifepristone for 72 h, the expression of Ki-67 antigen was declined and the amount of hCG secretion was decreased significantly at the third day.
In vitro, mifepristone has cytostatic and cytotoxic effects on JAR cell line and may inhibit the secretion of hCG in JAR cells.
探讨米非司酮对人绒毛膜癌细胞系JAR细胞增殖及人绒毛膜促性腺激素（hCG）分泌的影响，以及米非司酮对该细胞系的细胞毒性作用。
采用甲基噻唑基四氮唑（MTT）比色法评估四种不同浓度（1、5、10、20 μmol/L）米非司酮的细胞毒性作用。分别采用抗生物素蛋白-生物素复合物法、免疫组织化学法及酶联免疫吸附测定（ELISA）法检测JAR细胞中核增殖抗原Ki-67的表达及hCG分泌量。
四种不同浓度的米非司酮对JAR细胞均有剂量依赖性细胞毒性作用，在20 μmol/L时达到平台期。当JAR细胞暴露于5 μmol/L米非司酮72小时时，Ki-67抗原的表达在第三天下降，hCG分泌量显著减少。
在体外，米非司酮对JAR细胞系具有细胞生长抑制和细胞毒性作用，可能抑制JAR细胞中hCG的分泌。
