Hong Y, Lou Y J
Department of Pharmacology, School of Pharmacy, Zhejiang University, Hangzhou 310006, China.
Acta Pharmacol Sin. 2000 Jun;21(6):554-6.
To seek a sensitive time point for pre-gastrulation embryos exposed to developmental toxic agents, and to establish a molecular biomarker to evaluate the mechanism of cyclophosphamide-induced embryonic abnormalities in vivo.
Pregnant rats on d 3 of gestation were given i.p. cyclophosphamide (Cyc) 10, 20, 40 mg.kg-1. SDS-PAGE was performed to qualitatively observe the target proteins in d 8 rat embryos.
The expression of the protein with a molecular weight (M(r)) of approximately 70 kDa distinctively increased and that of the blastocyst-specific protein (M(r) 14.4 kDa) disappeared in Cyc 40 mg.kg-1 group.
Day 8 of rat gestation could be an optimum time point for understanding developmental toxicity of mammalian embryo during pregastrulation, and the expression of the proteins with M(r) 70 kDa and 14.4 kDa at this point could be employed as a molecular biomarker to demonstrate embryoteratology objectively and sensitively.
寻找原肠胚形成前胚胎暴露于发育毒性剂的敏感时间点，并建立一种分子生物标志物以评估环磷酰胺在体内诱导胚胎异常的机制。
妊娠第3天的孕鼠腹腔注射10、20、40mg·kg-1的环磷酰胺（Cyc）。进行SDS-PAGE定性观察第8天大鼠胚胎中的靶蛋白。
在40mg·kg-1环磷酰胺组中，分子量（M(r)）约为70kDa的蛋白表达明显增加，而胚泡特异性蛋白（M(r)14.4kDa）的表达消失。
大鼠妊娠第8天可能是了解哺乳动物胚胎原肠胚形成前发育毒性的最佳时间点，此时分子量为70kDa和14.4kDa的蛋白表达可作为分子生物标志物，客观、灵敏地证明胚胎毒理学。
