Lindback T, Frantz T
Acta Obstet Gynecol Scand. 1975;54(2):101-3. doi: 10.3109/00016347509156740.
Amniotic fluid lecithin and sphingomyelin were determined quantitatively in 33 samples obtained throughout the last trimester. Each sample was divided into three parts and each part was centrifuged at different relative forces prior to extraction. It is shown that centrifugation always removes considerable amounts of both lecithin and sphingomyelin from the supernatant towards the end of pregnancy, but very little at the beginning of the last trimester. The fatty acid composition of amniotic fluid lecithin, studied by gas liquid chromatography, indicate that surface active lecithin is lost by centrifugation of the fluid prior to extraction.
在妊娠晚期获取的33份样本中对羊水卵磷脂和鞘磷脂进行了定量测定。每个样本被分成三份，在提取前每份以不同的相对离心力进行离心。结果显示，在妊娠末期，离心总是会使相当数量的卵磷脂和鞘磷脂从上清液中转移至沉淀物中，但在妊娠晚期开始时这种情况很少发生。通过气相色谱法研究羊水卵磷脂的脂肪酸组成表明，在提取前对羊水进行离心会导致具有表面活性的卵磷脂流失。
