Ma B, Yi B D, Xing B R
Department of Physiology, Second Military Medical University, Shanghai 200433.
Sheng Li Xue Bao. 1998 Dec;50(6):679-82.
In the present study, the rapid effect of hydrocortisone 21-hemisuccinate (F-suc) on the amplitude of f-EPSP of B cells in isolated bullfrog sympathetic ganglia was examined with the use of intracellular recording technique. It was found that the amplitude of f-EPSP of B cells was decreased by F-suc, with a latency less than 3 min after the beginning of perfusion of F-suc. The inhibition of F-suc was concentration-dependent and could be blocked by the antagonist of intracellular glucocorticoid receptor RU38486, but not by the protein synthesis inhibitor actidione. These findings suggest that the inhibition of f-EPSP of B cells by F-suc is probably mediated through a nongenomic mechanism.
在本研究中，运用细胞内记录技术检测了氢化可的松21-半琥珀酸酯（F-suc）对离体牛蛙交感神经节中B细胞场兴奋性突触后电位（f-EPSP）幅度的快速作用。结果发现，F-suc可使B细胞的f-EPSP幅度降低，在开始灌注F-suc后潜伏期小于3分钟。F-suc的抑制作用呈浓度依赖性，且可被细胞内糖皮质激素受体拮抗剂RU38486阻断，但不能被蛋白质合成抑制剂放线菌酮阻断。这些发现提示，F-suc对B细胞f-EPSP的抑制作用可能是通过非基因组机制介导的。
