[一种新的大鼠脑胶质瘤模型]
[A new rat brain-glioma model].
作者信息
Xing B, Ren Z, Su C
机构信息
PUMC Hospital, CAMS and PUMC, Beijing 100730.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1998 Aug;20(4):241-5.
OBJECTIVE
To build a new rat brain glioma model with LacZ as a reporter gene.
METHODS
Plasmid BAG was transfected into C6 glioma cell. The cell cycle, cell proliferation, malignant infiltrative ability of wild and transfected glioma cell were observed. Rat glioma model was built using stereotaxic implantation.
RESULTS
There was no difference between transfected and wild glioma cell in cell growth characteristics and tumorigenesis.
CONCLUSIONS
It was easy to observe the infiltrative area on cellular level from the rat glioma model made by transfected tumor cell. The method is satisfactory for radiation and chemotherapeutic trials of glioma.
目的
构建以LacZ作为报告基因的新型大鼠脑胶质瘤模型。
方法
将质粒BAG转染至C6胶质瘤细胞。观察野生型和转染后胶质瘤细胞的细胞周期、细胞增殖及恶性浸润能力。采用立体定向植入法建立大鼠胶质瘤模型。
结果
转染后的胶质瘤细胞与野生型胶质瘤细胞在细胞生长特性及致瘤性方面无差异。
结论
由转染后的肿瘤细胞制成的大鼠胶质瘤模型易于在细胞水平观察浸润区域。该方法适用于胶质瘤的放射治疗和化学治疗试验。
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