Osterreicher J, Mokry J, Navrátil L, Knízek J, Vávrová J, Skopek J, Macela A
Department of Radiobiology and Immunology, Purkynĕ Military Medical Academy, Hradec Králové.
Acta Medica (Hradec Kralove). 2001;44(1):15-9.
We studied the relationship between type II pneumocytes number and alveolar septal thickness during different time after sublethal whole-thorax irradiation of rats and we investigated the influence of pentoxifylline (TNF-alpha inhibitor).
Wistar rats were exposed to 15 Gy thoracic irradiation and pentoxifylline (35 mg/kg) twice a week. Lungs were examined histologically and immunohistochemically at intervals ranging from 1-12 weeks and alveolar septal thickness, number of type II pneumocytes (identified by immunoreactivity for cytokeratin 18), and neutrophile granulocytes were counted.
Significant increase of alveolar septal thickness and type II pneumocytes depletion 3 weeks after irradiation were found. Correlation of these markers was r = -0.759. Pentoxifylline significantly inhibits increased alveolar septal thickness without the influence on type II pneumocytes number. Neutrophil penetration started 5 weeks after irradiation in non-treated animals, 8 weeks after irradiation in PTX-treated rats.
We suggest that pneumocytes depletion is linked to increased vascular permeability, and pentoxifylline therapy does not influence on pneumocytes kinetics after irradiation.
我们研究了大鼠亚致死性全胸照射后不同时间点II型肺细胞数量与肺泡间隔厚度之间的关系，并研究了己酮可可碱（肿瘤坏死因子-α抑制剂）的影响。
将Wistar大鼠暴露于15 Gy胸部照射，并每周两次给予己酮可可碱（35 mg/kg）。在1至12周的间隔时间对肺进行组织学和免疫组织化学检查，并计算肺泡间隔厚度、II型肺细胞数量（通过细胞角蛋白18免疫反应性鉴定）和中性粒细胞数量。
照射后3周发现肺泡间隔厚度显著增加和II型肺细胞减少。这些指标的相关性为r = -0.759。己酮可可碱显著抑制肺泡间隔厚度增加，而不影响II型肺细胞数量。未治疗动物在照射后5周开始出现中性粒细胞浸润，己酮可可碱治疗的大鼠在照射后8周出现。
我们认为肺细胞减少与血管通透性增加有关，己酮可可碱治疗对照射后肺细胞动力学无影响。
