Royer A, Ménand M, Grimault A, Communal P Y
Groupement Interregional de Recherche sur les Produits Agropharmaceutiques, Angers Technopole, 8, rue H. Becquerel, 49070 Beaucouzé, France.
J Agric Food Chem. 2001 May;49(5):2152-8. doi: 10.1021/jf0013196.
The determination of dithiocarbamates in plant matrixes is generally carried out by spectrophotometric (European Norm EN 12396-1, 1996) or gas chromatography headspace (European Norm EN 12396-2, 1999) methods. However, the former method presents a risk of carbon disulfide loss during hydrolysis and distillation and its sensitivity is low, whereas the latter method is time-consuming. In comparison to these European methods and in compliance with norm V03-110, we have developed an automated gas chromatography headspace method. This method offers a good level of accuracy and precision and is specific to the compound determined (CS(2)). The limit of detection is below 0.020 mg/kg and the limit of quantification is below 0.050 mg/kg. Moreover, the recovery rates are between 85 and 103% with RSD less than 20%. The automated headspace method has several advantages when compared to the spectrophotometric and manual headspace methods, including the reduction of reagents employed for extraction and a greater number of analyses achievable per day than the other methods (approximately 40 samples of food).
植物基质中二硫代氨基甲酸盐的测定通常采用分光光度法（欧洲标准EN 12396-1，1996）或气相色谱顶空法（欧洲标准EN 12396-2，1999）进行。然而，前一种方法在水解和蒸馏过程中存在二硫化碳损失的风险，且灵敏度较低，而后一种方法耗时较长。与这些欧洲方法相比，并符合V03-110标准，我们开发了一种自动气相色谱顶空法。该方法具有良好的准确度和精密度，且对所测定的化合物（CS(2)）具有特异性。检测限低于0.020 mg/kg，定量限低于0.050 mg/kg。此外，回收率在85%至103%之间，相对标准偏差小于20%。与分光光度法和手动顶空法相比，自动顶空法具有多个优点，包括减少了提取所用的试剂，并且每天可完成的分析数量比其他方法更多（约40个食品样品）。
