Kakitani Ayano, Yoshioka Toshiaki, Nagatomi Yasushi, Harayama Koichi
Department of Analytical Technology Development, Food Safety Laboratories, Asahi Group Holdings, Ltd.
J Pestic Sci. 2017 Nov 24;42(4):145-150. doi: 10.1584/jpestics.D17-025.
We herein present a method for the analysis of 10 dithiocarbamate fungicides (DTCs) in beer, fruit juice, and malt samples, where the DTCs were converted into water-soluble sodium salts in the presence of NaHCO prior to methylation using dimethyl sulfate. Extraction of the methylated compounds from matrices was performed using a "quick, easy, cheap, effective, rugged, and safe" (QuEChERS) method. Following a dispersive solid-phase extraction as a clean-up step, the methylated compounds were detected by liquid chromatography-tandem mass spectrometry. Performance evaluation was carried out on beer, fruit juice, and malt samples using representative compounds. Accuracies of the spiked compounds from the various matrices ranged from 92.2 to 112.6%, and the limits of quantification of propineb, mancozeb, and thiuram were <0.52, <0.55, and <6.97 µg/kg, respectively. The developed method was then applied in the determination of dithiocarbamate fungicide contents in commercial beer and fruit juice samples.
我们在此介绍一种分析啤酒、果汁和麦芽样品中10种二硫代氨基甲酸盐类杀菌剂（DTCs）的方法，其中在使用硫酸二甲酯进行甲基化之前，DTCs在NaHCO存在下转化为水溶性钠盐。使用“快速、简便、廉价、高效、耐用和安全”（QuEChERS）方法从基质中提取甲基化化合物。作为净化步骤，在进行分散固相萃取后，通过液相色谱 - 串联质谱法检测甲基化化合物。使用代表性化合物对啤酒、果汁和麦芽样品进行性能评估。来自各种基质的加标化合物的回收率在92.2%至112.6%之间，代森锌、代森锰锌和福美双的定量限分别<0.52、<0.55和<6.97µg/kg。然后将所开发的方法应用于测定市售啤酒和果汁样品中二硫代氨基甲酸盐类杀菌剂的含量。
