Sirato-Yasumoto S, Katsuta M, Okuyama Y, Takahashi Y, Ide T
National Food Research Institute, Ministry of Agriculture Forestry and Fisheries, 2-1-2 Kannondai, Tsukuba 305-8642, Japan.
J Agric Food Chem. 2001 May;49(5):2647-51. doi: 10.1021/jf001362t.
Activities of enzymes involved in hepatic fatty acid oxidation and synthesis among rats fed sesame (Sesamum indicum L.) differing in lignan content (sesamin and sesamolin) were compared. Sesame seeds rich in lignans from two lines, 0730 and 0732, lines established in this laborary, and those from a conventional cultivar (Masekin) were employed. Seeds from the 0730 and 0732 lines contained sesamin and sesamolin at amounts twice those from Masekin. Sesame seeds were added at levels of 200 g/kg to the experimental diets. Sesame increased both the hepatic mitochondrial and the peroxisomal fatty acid oxidation rate. Increases were greater with sesame rich in lignans than with Maskin. Noticeably, peroxisomal activity levels were >3 times higher in rats fed diets containing sesame seeds from the 0730 and 0732 lines than in those fed a control diet without sesame. The diet containing Masekin seed caused only a 50% increase in the value, however. Diets containing seeds from the 0730 and 0732 lines, compared to the control and Masekin diets, also significantly increased the activity of hepatic fatty acid oxidation enzymes including acyl-CoA oxidase, carnitine palmitoyltranferase, 3-hydroxyacyl-CoA dehydrogenase, and 3-ketoacyl-CoA thiolase. In contrast, diets containing sesame lowered the activity of enzymes involved in fatty acid synthesis including fatty acid synthase, glucose-6-phosphate dehydrogenase, ATP-citrate lyase, and pyruvate kinase. No significant differences in enzyme activities were, however, seen among diets containing sesame from Masekin cultivar and lines 0730 and 0732. Serum triacylglycerol concentrations were lower in rats fed diets containing sesame from lines 0730 and 0732 than in those fed the control or Masekin diet. It is apparent that sesame rich in lignans more profoundly affects hepatic fatty acid oxidation and serum triacylglycerol levels. Therefore, consumption of sesame rich in lignans results in physiological activity to alter lipid metabolism in a potentially beneficial manner.
比较了喂食不同木脂素含量（芝麻素和芝麻林素）芝麻（Sesamum indicum L.）的大鼠肝脏中参与脂肪酸氧化和合成的酶的活性。使用了本实验室培育的两个品系0730和0732中富含木脂素的芝麻种子，以及一个传统品种（牧之锦）的芝麻种子。0730和0732品系的种子中芝麻素和芝麻林素的含量是牧之锦种子的两倍。芝麻种子以200 g/kg的水平添加到实验日粮中。芝麻提高了肝脏线粒体和过氧化物酶体的脂肪酸氧化速率。富含木脂素的芝麻的提高幅度大于牧之锦芝麻。值得注意的是，喂食含有0730和0732品系芝麻种子日粮的大鼠的过氧化物酶体活性水平比喂食不含芝麻的对照日粮的大鼠高3倍以上。然而，含有牧之锦种子的日粮仅使该值增加了50%。与对照日粮和牧之锦日粮相比，含有0730和0732品系种子的日粮还显著提高了肝脏脂肪酸氧化酶的活性，包括酰基辅酶A氧化酶、肉碱棕榈酰转移酶、3-羟基酰基辅酶A脱氢酶和3-酮酰基辅酶A硫解酶。相反，含有芝麻的日粮降低了参与脂肪酸合成的酶的活性，包括脂肪酸合酶、葡萄糖-6-磷酸脱氢酶、ATP-柠檬酸裂解酶和丙酮酸激酶。然而，在含有牧之锦品种以及0730和0732品系芝麻的日粮之间，未观察到酶活性的显著差异。喂食含有0730和0732品系芝麻日粮的大鼠的血清三酰甘油浓度低于喂食对照日粮或牧之锦日粮的大鼠。显然，富含木脂素的芝麻对肝脏脂肪酸氧化和血清三酰甘油水平的影响更为深远。因此，食用富含木脂素的芝麻会产生生理活性，以潜在有益的方式改变脂质代谢。
