Bollmann R
Institut für Pathologie, Bonn.
Zentralbl Gynakol. 2001 Apr;123(4):206-10. doi: 10.1055/s-2001-14776.
The diagnostic group of dysplasias has been described as "a group of diagnostic impotence". DNA-aneuploidy detected by image cytometry of Feulgen stained pap smears indicates a potentially progressive lesion, representing a high-grade lesion. DNA-cytometry can identify cases of dysplasias which are likely to progress: DNA-aneuploid dysplasias are HSIL. DNA-aneuploidy is the expression of an integral HPV infection. CIN-lesions with episomal HPV infections are DNA-diploid. With the newly developed laserscanning cytometer (LSC) of ThinPrep-Preparation an automatisation of ploidy measurement is possible in combination with HPV-PCR. We recommend DNA-Image-cytometry as a routine method for classification of uterine cervical borderline lesion into regressive and progressive.
发育异常的诊断类别被描述为“一组诊断上的难题”。通过对福尔根染色巴氏涂片进行图像细胞计量术检测到的DNA非整倍体表明存在潜在进展性病变,代表高级别病变。DNA细胞计量术可识别可能进展的发育异常病例:DNA非整倍体发育异常为高级别鳞状上皮内病变(HSIL)。DNA非整倍体是完整人乳头瘤病毒(HPV)感染的表现。伴有游离型HPV感染的宫颈上皮内瘤变(CIN)病变为DNA二倍体。使用新开发的ThinPrep制片的激光扫描细胞仪(LSC),结合HPV聚合酶链反应(PCR)可实现倍性测量的自动化。我们推荐将DNA图像细胞计量术作为将子宫颈交界性病变分类为退行性和进展性病变的常规方法。