Al-Awadhi Rana, Husain Sawsan, Chehadeh Wassim, Al-Jassar Waleed, Kapila Kusum, Al-Mulla Fahd
Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, Kuwait City, Kuwait.
Acta Cytol. 2013;57(3):259-65. doi: 10.1159/000350249. Epub 2013 Apr 25.
This study investigates the potential value of Raf kinase inhibitor protein (RKIP) as a marker of normal squamous cells in ThinPrep slides. RKIP was evaluated for its ability to distinguish between normal and abnormal cervical samples in the context of human papillomavirus (HPV) infections.
A total of 316 ThinPrep samples were taken from women with normal and abnormal cervices. ThinPrep slides were Papanicolaou stained and reported. Residual samples were used for RKIP immunostaining and HPV PCR-based sequencing.
RKIP expression was seen in both nuclei and cytoplasm in 83.7% of samples. RKIP expression was highest (84.6%) in samples with a diagnosis of high-grade squamous intraepithelial lesion (HSIL) or worse; expression was lower in low-grade squamous intraepithelial lesions (73%) and was lowest in samples with normal cytology (p = 0.0023). A total of 74% of HPV-infected ThinPrep samples were immunopositive, and 67% of samples that did not harbor HPV were also immunopositive (p = 0.414). Sensitivity and specificity of RKIP were 84.6 and 34.6%, respectively, for the detection of samples with HSIL or worse.
This study showed that RKIP expression may be of some value as a marker for abnormal cervical cells. Combined RKIP expression and HPV testing could improve the identification of samples with abnormal cytology.
本研究调查了Raf激酶抑制蛋白（RKIP）作为薄层液基制片（ThinPrep）玻片上正常鳞状细胞标志物的潜在价值。在人乳头瘤病毒（HPV）感染的背景下，评估了RKIP区分正常和异常宫颈样本的能力。
从宫颈正常和异常的女性中总共采集了316份ThinPrep样本。对ThinPrep玻片进行巴氏染色并报告结果。剩余样本用于RKIP免疫染色和基于HPV PCR的测序。
83.7%的样本在细胞核和细胞质中均可见RKIP表达。在诊断为高级别鳞状上皮内病变（HSIL）或更严重病变的样本中，RKIP表达最高（84.6%）；在低级别鳞状上皮内病变中表达较低（73%），在细胞学正常的样本中表达最低（p = 0.0023）。总共74%的HPV感染的ThinPrep样本免疫阳性，67%未携带HPV的样本也免疫阳性（p = 0.414）。对于检测HSIL或更严重病变的样本，RKIP的敏感性和特异性分别为84.6%和34.6%。
本研究表明，RKIP表达作为宫颈异常细胞的标志物可能具有一定价值。联合RKIP表达和HPV检测可提高对细胞学异常样本的识别。
